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However, the literature suggests (5) that viremia in dengue lasts for less than 5 days and that the Ig M antibody response takes 5 to 10 days to develop in cases of primary dengue virus infection and 4 to 5 days in cases of secondary dengue virus infection (2, 5).Hence, at a particular time postinfection, it is unlikely that the results for both of these tests will be positive.
Previous studies (1, 3) have reported that NS1 antigen is positive for 9 days postinfection in cases of primary dengue and up to 5 days postinfection in cases of secondary dengue.The results for the NS1 test were positive for an additional 15 samples which were negative by conventional RT-PCR (4).It is important to know that NS1 testing is also available as a strip test and also as a dengue duo test (NS1/Ig M/Ig G), which has further increased the sensitivity of detection.However, if a patient has had dengue before, this test can yield positive results even before 5 days.Both the tests that have been mentioned can be done without prior processing of the patients’ blood. whole blood can be used) In addition to the tests mentioned thus far, there are other investigations to confirm dengue. Both the NS1 antigen test and the dengue antibody test are very accurate.Admin Street: 202 South Minesota Street Admin City: Cusron City Admin State/Province: NV Admin Postal Code: 89703 Admin Country: US Admin Phone: 1.3102036699 Admin Phone Ext: Admin Fax: Admin Fax Ext: Admin Email: @37Registry Tech ID: tu Vd Xr5IBo7Op Sz K Tech Name: Hostmaster Hostmaster Tech Organization: USGO, Inc.
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We suggest that NS1 antigen should be considered the test of choice for patients presenting with a history of fever of up to 3 days of duration.
On day 4, a combination of MAC-ELISA and an NS1 antigen test would increase the sensitivity of diagnosis.
It is important to know in how many cases the virus genome could be detected until day 8 out of the 50 samples that were positive by real-time PCR.
It would also be valuable to know whether the same patient's blood was drawn for 8 consecutive days and checked for viremia and, if so, whether the virus was detectable.
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